Several state and regionally rare species rely on Pioneer Park for habitat, including belted kingfishers, American redstarts, yellow-billed cuckoos, red-headed woodpeckers, ovenbirds, and breeding Cooper’s hawks, downy woodpeckers, and white-breasted nuthatches. Ecologists have identified beavers, coyotes, American toads, water and queen snakes, and a variety of freshwater mussels and aquatic invertebrates at the forest preserve as well.
Access the DuPage River Water Trail from Pioneer Park for canoeing and kayaking. Pioneer Park is also a great spot for fishing in the DuPage River. The West Branch DuPage River is home to smallmouth and largemouth bass, crappie, and channel catfish.