In addition to wetlands and upland oak savannas, the preserve is home to one of the most biologically diverse sites in northeastern Illinois, the 126-acre Truitt-Hoff Nature Preserve. It is one of eight state-designated nature preserves in DuPage County owned and operated by the Forest Preserve District. Because the Truitt-Hoff Nature Preserve and other nature preserve land covers all of West Chicago Prairie Forest Preserve, in accordance with the Illinois Natural Areas Preservation Act, bicycles and horses are not allowed at the forest preserve.
Nine linking footpaths meander through West Chicago Prairie. Many connect with the Illinois Prairie Path, which leads outside of the forest preserve and connects with West Chicago’s Reed-Keppler Park.